Characterization of Acid Sphingomyelinase Activity in Human Cerebrospinal Fluid

Characterization of Acid Sphingomyelinase Activity in Human Cerebrospinal Fluid

BACKGROUND As a key enzyme in sphingolipid metabolism, acid sphingomyelinase (ASM) is involved in the regulation of cell fate and signaling via hydrolysis of sphingomyelin to form ceramide. While increased activity of the lysosomal form has been associated with various pathological conditions, there are few studies on secretory ASM limited only to cell models, plasma or serum. METHODS An opti...

Ouabain-like activity in human cerebrospinal fluid.

Human cerebrospinal fluid has been found to mimic the effect of ouabain on net Na+ efflux and 86Rb+ influx across erythrocyte membranes and on the in vitro activity of a purified Na+/K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) derived from canine kidney. These results indicate the possible existence in human cerebrospinal fluid of an endogenous factor with ouabain-like activity, which might be...

Ascorbic Acid in Cerebrospinal Fluid.

Estimation of Lactate Dehydrogenase Activity in cerebrospinal fluid in different types of meningitis

Objective: Meningitis is life-threatening condition and examination of the Cerebrospinal fluid (CSF) may not give a precise diagnosis and prognosis of different types of meningitis. Bacterial meningitis is still a very common problem especially in many developing countries. The aims of study are to estimate the importance and significance of Lactate Dehydrogenase (LDH), protein...

The ontogeny of 3,4-dihydroxyphenylacetic acid in human cerebrospinal fluid.

Measurement of cerebrospinal fluid (CSF) levels of the minor dopamine metabolite 3,4-dihydroxyphenylacetic acid in neonates and children indicates that a rapid decline (approximately 90%) occurs in the first 2 years of life. The much less rapid ontogenetic decline seen for the predominant dopamine metabolite, homovanillic acid (HVA), indicates that differing factors affect CSF levels of the two...